青霉素V钾滴丸的研制

为减少青霉素V钾的水解并提高其疗效，用聚乙二醇400和聚乙二醇6000为基质制备青霉素V钾滴丸。体外抑菌试验表明，青霉素v钾滴丸抑菌作用略强于人药片剂。     

VA菌根对茄子、黄瓜、籽瓜促生防病效应的研究

在盆栽条件下研究了ＶＡ菌根茵对茄子、黄瓜、籽瓜的侵染、促生和防病作用。结果供试菌均可侵染供试作物，但促生和防病效应具特异性．Ｇｌｏｍｕｓ．Ｖｅｒｓｉｆｏｒｍｅ和Ｇ．ｍｏｓｓｅａｅ均促进茄子的生长，但只前者减轻了茄子黄萎病。Ｇ，ｓｐ３促进黄瓜提早开花，但不防病；Ｇ．ｍａｃｒｏｃａｒｐｕｍ减轻了黄瓜细菌性角斑病的发生，却并无促生作用。Ｇ．ｓｐ４不但促进籽瓜的生长，而且是短期内减轻籽瓜炭疽病的有效组合。     

中国野生种毛葡萄光合特性的研究

毛葡萄（ＶｉｔｉｓｑｕｉｎｑｕａｎｇｕｌａｒｉｓＲｅｈｄ）的净光合速率日变化值明显高于山葡萄、宝石、白玉霓，且无栽培品种光合作用的“午休”现象。毛葡萄的净光合速率与其它野生种葡萄和栽培品种比较也表现出较高水平。在进行ＣＯ２饱和点和补偿点测定时，毛葡萄也较栽培品种白玉霓有较强的光合作用，因此，毛葡萄是葡萄高光合效率育种的优良野生种质资源。     

The intravenous bile Acid loading test in the goat: determination of a procedural protocol and reference values

An intravenous bile acid loading test was performed on 16 goats using various dosages and sampling protocols. A recently developed enzymatic bile acid assay provided an economical and technologically simple method for bile acid analysis. Intravenous injection of bile salts into goats was well tolerated. Clearance times were rapid. A protocol for performance of the test was suggested and a practical reference range for T 1/2 clearance of 4+/-1 minute was determined. Further investigations of this test in the assessment of liver function in the ruminant appear warranted.     

Contribution to the taxonomy and pathogenicity of fungicolous Verticillium species. II. Pathogenicity

In recent years in the Netherlands a second mushroom species,Agaricus bitorquis, which prefers higher temperatures thanA. bisporus and is less susceptible to certain diseases, is often commercially grown.Verticillium fungicola var.fungicola, the causal agent of dry bubble, is responsible for considerable damage in crops ofA. bisporus. InA. bitorquis, however, dry bubble has hardly been noticed, but brown spots due toV. fungicola var.aleophilum resulted in inferior mushroom quality. The latter variety also caused brown spots ina. bisporus, but to a minor degree. In variety Les Miz 60 ofA. bisporus, however, it also induced fruit-body deformation in a way different from dry bubble. Verticillium psalliotae, isolated fromA. bitorquis in England, induced more confluent brown spots inA. bitorquis. In the netherlands, where moreA. bitorquis is grown than in other countries,V. psalliotae has not yet been encountered in crops ofA. bitorquis. V. psalliotae, which has a high temperature optimum for mycelial growth, likeV. fungicola var.aleophilum andA. bitorquis, did not infectA. bisporus in our trials.Artificial infection ofA. bisporus orA. bitorquis could not be accomplished with the following related and/or fungicolous fungi:Verticillium lamellicola, V. fungicola var.flavidum, V. biguttatum, Nectriopsis tubariicola, Acremonium crotocinigenum andAphanocladium album.Samenvatting Vooral in Nederland wordt sinds een aantal jaren naastAgaricus bisporus ook de warmteminnende champignonsoortAgaricus bitorquis geteeld, die minder vatbaar is voor bepaalde ziekten. TerwijlVerticillium fungicola varfungicola in de teelt vanA. bisporus droge mollen en daardoor veel schade veroorzaakt, komen in de teelt vanA. bitorquis geen droge mollen voor maar wel bruine vlekken, die tot kwaliteitsverlies en dus schade leiden. De vlekken bleken veroorzaakt te worden doorV. fungicola var.aleophilum. Deze schimmel veroorzaakte ook inA. bisporus bruine vlekken, hoewel in minder ernstige mate, maar in het ras Les Miz 60 vanA. bisporus bovendien misvormde champignons, die wel op droge mollen leken, maar daaraan niet gelijk waren.OokV. psalliotae, in Engeland geïsoleerd vanA. bitorquis met vlekken, veroorzaakte wat meer samenvloeiende, bruine vlekken inA. bitorquis. In Nederland, waar meerA. bitorquis geteeld wordt dan in andere landen, isV. psalliotae nog niet aangetroffen in teelten vanA. bitorquis. InA. bisporus kon geen kunstmatige infectie worden verkregen metV. psalliotae, die net alsV. fungicola var.aleophilum enA. bitorquis warmteminnend genoemd zou kunnen worden.Met de volgendeVerticillium-achtige of van paddestoelen geïsoleerde schimmels kon evenmin op kunstmatige wijze een infectie worden opgeroepen inA. bisporus ofA. bitorquis: Verticillium lamellicola, V. fungicola var.flavidum, V. biguttatum, Nectriopsis tubariicola, Acremonium crotocinigenum enAphanocladium album.     

外源甜菜碱对作物的抗旱作用效果研究

通过小麦浸种、水培、大面积喷施和茄子盆栽试验,研究了不同浓度外源甜菜碱对作物的抗旱作用效果。结果表明:外源甜菜碱浓度为25～50mg/L时可促进小麦种子发芽,光合作用强度[达7.07～7.57μmol/(s·m~2)]及生物学产量增加;在外源甜菜碱浓度100 mg/L时小麦幼苗叶片硝酸还原酶活性最高,达到35.23μg/(g·h),同时小麦抗旱性增强。数学模式寻优结果表明:在外源甜菜碱浓度26.4～68.9mg/L,土壤水分含量12.6％～20.7％时,可获得最佳的茄子生物总量。     

Suppression of Verticillium Wilt in Eggplant by Some Fungal Root Endophytes

One hundred and twenty-three fungal isolates were obtained from 225 root segments of eggplants, melon, tomato, strawberry and Chinese cabbage, grown as bait plants in a mixed soil made up of samples from different fields in Shizuoka, Japan. Isolates belonging to Mycelium radicis atrovirens (MRA), including Phialocephala fortinii, were the most prevalent in all the five bait plants. Eleven of the 123 isolates, after being inoculated onto axenically reared eggplant seedlings, almost completely suppressed the pathogenic effects of a post-inoculated, virulent strain of Verticillium dahliae. Seven of these 11 isolates had come from the roots of eggplant and included Heteroconium chaetospira, P. fortinii, and unidentified species of Fusarium, Penicillium, Trichoderma and MRA. P. fortinii, H. chaetospira, a non-sporulating isolate with white mycelium (SWM) and MRA were easily reisolated from root segments. Hyphae of H. chaetospira, P. fortinii and SWM colonized the root tissues of eggplant without causing apparent pathogenic symptoms. The mechanisms by which these endophytes confer resistance to infection by V. dahliae are unknown but the effectiveness of these fungi in a laboratory setting indicates that they have potential as biocontrol agents and merit further investigation.     

IL-12 induces T lymphocytes apoptosis and influences the expression and signal transduction of Fas/FasL

AIM: IL-12 acts upon Tlymphocytes and activates its receptor complexes of β1/β2,and so IL-12 can regulate TH1/TH2 balance. Our study is aimed at IL-12-inducing apoptosis of Tcells and expression and signal transduction of Fas/FasLduring Tcell apoptosis. METHODS: The apoptosis of Tcells was detected by Annexin Vstaining cytometry and the expression of Fas/FasLunder different inhibitors were detected by semi-quantitative PCR. RESULTS: IL-12 induced the human leukemic Tcell line(TIB-152) and the human lymphoma Tcell line(HTB-176) and the normal human Tcells to undergo apoptosis. The FasLexpression at 6 hours after treatment with IL-12 increased apparently, and reached the max at 24 hours, and FasLexpression induced by IL-12 was inhibited by PKCinhibitor. But IL-12 did not influence Fas expression. CONCLUSIONS: IL-12 can induce Tcells to undergo apoptosis which is characterized by early membrane changes, the inducing effect is correlated with the concentration of IL-12 and the maturation of Tcells. FasLparticipates in the progression of Tcell apoptosis as a apoptosis mediator, and the effect of IL-12 on FasLexpression may be related with PKCpathway.     

茄子单性结实差异表达序列鉴定及分析

利用实时荧光定量PCR技术,研究茄子单性结实SSH-c DNA文库中的96条EST序列在单性结实自交系和非单性结实自交系果实发育过程中的表达模式。分析表明,在低温条件下,相对表达量有显著差异的EST序列有31条,总体上调表达的EST序列有17条,总体下调表达的EST序列有14条。通过NCBI对EST序列进行Blastx比对,得到与其同源性高的序列信息。其中5条EST与抵御低温相关,6条EST与植物激素代谢相关,多条EST与植物代谢过程中的蛋白质和碳水化合物合成相关,1条EST无比对结果,可能为新基因。差异表达序列可作为研究茄子单性结实和耐低温的候选基因。     

TRMM在海河流域南系的降水估算精度评价及其对SWAT模型的适用性

准确估算区域降水对水文过程评价和水资源管理意义重大。为评估TRMM 3B42V7降水产品在海河流域南系的估算精度及其在土壤和水评估模型(Soil and Water Assessment Tool,SWAT)中的适用性,利用28个气象站降水观测数据(2007-2016年)和101个雨量站观测数据(2010-2016年)开展研究。研究表明:站点尺度上,3B42V7降水产品对月降水估算的均方根误差小于15 mm,平均误差小于8.5 mm;在湿润季节的估算精度更好。流域尺度上,日降水估算精度较差,相关系数小于0.6。分区尺度上,3B42V7能够很好地捕捉到不同等级降水强度,但对微量降雨有所低估;山区和平原的年降水量均出现高估现象,平原区较为突出;此外,3B42V7能够较好地捕捉到研究区内极端降水的时间和空间分布。分2种情景进行水文模拟,利用月平均流量对模型进行校准和验证,在情景Ⅰ中,验证期模拟结果较好,决定系数在0.56~0.96之间,纳什效率系数在-11.09~0.94之间。TRMM 3B42V7可为海河流域及其类似区域的水资源管理提供参考。